INTRODUCTION TO BIOSCIENCES FOR FORENSIC INVESTIGATION

SHE Level 1
SCQF Credit Points 20.00
ECTS Credit Points 10.00
Module Code M1C725877
Module Leader Karen Keith
School School of Health and Life Sciences
Subject Biological and Biomedical Sciences
Trimester
  • A (September start)-B (January start)

Summary of Content

-2582 The module will deliver a core programme of biochemistry, cell biology and genetics with an emphasis on underpinning the Advanced Forensic Biology module. Topics covered will include the synthesis, structure and function of DNA, RNA and proteins, genetics covering mitosis and meiosis as well as models of inheritance and inherited disease. Enzyme biology covering thermodynamics and kinetics, mechanisms of action and allosteric enzymes will also be covered.

Syllabus

-2582 Membrane structure and function 6 hours -360-2582b7 The structure of lipids, phospholipids, carbohydrates, glycoproteins and lipoproteins -360-2582b7 Membrane structure models b7 Membrane as a barrier. b7 Transport across the membrane: diffusion and facilitated transport. b7 Osmosis Na/K pump. Proton pump bulk transport b7 Cell fibres and the cytoskeleton. Centromere, centrioles. -359-2582 Introduction to nucleic acids 8 hours -360-2582b7 Structure synthesis and replication of DNA and RNA -360-2582b7 The genetic code and protein synthesis -360-2582b7 Preparation & analysis of DNA b7 DNA sequencing (all methods including Next Generation) b7 Structure of Genes: Eukaryotes b7 RNA stability b7 Preparation & analysis of RNA -359-2582 -359-2582 Enzymes structure, function and kinetics 10 hours -360-2582b7 Protein purification and characterisation. -360-2582b7 Understand that enzymes are proteins which catalyse metabolic reactions. -360-2582b7 Identify the major groups of enzymes. b7 The effects of temperature, pH, enzyme and substrate concentration on enzyme-catalysed reactions b7 Can the rate of an enzyme-catalyzed reaction be defined in a mathematical way? b7 What equations define the kinetics of enzyme-catalyzed reactions? b7 How can enzymes be so specific? What are the magnitudes of enzyme-induced rate accelerations? b7 What role does transition-state stabilization play in enzyme catalysis? b7 Types of catalysis: example of an enzyme mechanism b7 Describe the effects of competitive and non-competitive inhibitors on the rate of enzyme activity. b7 How can enzymes control the rate of biochemical pathways? b7 What are the models of allosterism? b7 Aspartate carbamoylase as an example of an allosteric enzyme -2582 Genetics and inheritance: 6 hours -360-2582b7 Chromosome structure and function -360-2582b7 Composition and number of chromosomes -360-2582b7 Construction of a karyotype b7 The stages of the cell cycle including mitosis and meiosis -360-2582b7 Mendelian Genetics and Disease -360-2582b7 How Mendel constructed his experiments -360-2582b7 Difference between F1 and F2 generations -360-2582b7 The Model of Heredity including test, monohybrid and dihybrid crosses b7 Construction of a genetic map b7 The concept of multiple alleles b7 The consequences of alterations in chromosome number b7 Inheritance of autosomal dominant / recessive and sex-linked disorders

Learning Outcomes

On successful completion of the module the student should be able to:1. Name the key molecules and pathways that constitute the central dogma including the structure and function of DNA, RNA and protein (Ex01).2. Define the nature of cell membranes and transport of molecules across membranes (Ex01).3. Recognise the basis of genetic inheritance (Ex01).4. Describe the nature & function of enzymes their substrates, modulators and inhibitors (Ex01).5. Demonstrate laboratory skills appropriate to the programme of experimental work (CW01).6. Record, analyse and present experimental data in graph and table format (CW01).

Teaching / Learning Strategy

Material will be delivered by lectures and supported by material presented in tutorials. Online, audio-visual, multimedia resources, and textbooks will be used to reinforce and underpin the laboratory programme. Students will work on a laboratory portfolio which will be developed as they progress through the module. Three practical classes per semester will support the learning outcomes.

Indicative Reading

-2582 Cells, Molecules and Metabolism, GCU Department of Life Sciences, Wiley Custom Text Book (2017) Essential Cell Biology, Alberts, Hopkin, Johnson, Morgan, Raff, Roberts and Walter (2019) Garland Press Practical Skills in Biomolecular Sciences, 5th Edition. Reed, R., Holmes, D., Weyes, J. and Jones, A. (2016) Pearson /Prentice Hall

Transferrable Skills

The student will develop both laboratory and personal transferable (PT) skills. Transferable skills such as effective written communication and the presentation and analysis of data are integral components of the module. The laboratory programme will ensure that students can work both independently and as effective members of small groups to generate significant data. Students will consolidate core analytical bioscience skills such as pipetting and spectrophotometry and apply these skills to a range of biological issues.

Module Structure

Activity Total Hours
Lectures (FT) 30.00
Assessment (FT) 10.00
Independent Learning (FT) 132.00
Practicals (FT) 18.00
Tutorials (FT) 10.00

Assessment Methods

Component Duration Weighting Threshold Description
Coursework 1 n/a 40.00 35% Lab report portfolio with data analysis.
Exam (Exams Office) 2.00 60.00 35% Unseen written exam - MCQ and short answer questions.